1.
Comparision Of Synchronization Protocols On Estrus, Follicular Dynamics, Ovulation Time And Conception Rate in Sahiwal Cows
by Mubbashar Hassan | Prof. Dr. Nasim Ahmad | Dr. Aijaz Ali Channa | Dr. Aneela.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Sahiwalcow is one of the established milch breed of zebu cattle (Bosindicus), known for heat tolerance and tick resistance in tropical and subtropical region which has its origin in Pakistan.Average milk yield is about 1500-2200 liters per lactation with 4.5% contents of fat (Shah, 1994).The population of Sahiwal cattle in Pakistan is decreasing over the past few decades due to change in agriculture system, indiscriminate crossbreeding and preference for buffalo. To minimize the cross breeding of Sahiwal cows, efforts have been made for its conservation, through management and genetic improvement. Significant genetic improvement, through artificial insemination(A.I) has been made. Fixed time artificial insemination (FTAI) has emerged in recent times which usually performed in association with a variety of estrus and ovulation synchronization protocols.Synchronization of estrus is actually the manipulation of estrous cycle or induction of estrus to bring large group of females in estrus at a programmed time. Three synchronization protocols were compared. In PG group, each animal was treated with luteolytic dose of PGF2? (d-Cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m) on random stage of the estrous cycle. In OVS group each cow received an intramuscular injection of gonadotropin releasing hormone (GnRH; 50 mcg of a GnRH analogue, DalmarelinTMFatro®, Italy; 2 ml; i.m) on random stage of estrus cycle (day 0). On day 7 these cows were treated with PGF2? (d- cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m), followed by second injection of GnRH on day 9. In CIDR group, each animal received an intravaginal Controlled Internal Drug Releasing Device (CIDR™; 1.38 g progesterone Eazibreed™; InterAg, Hamilton, New Zealand) on random stage of estrus cycle that was considered as day 0 of treatment. CIDR was removed on day 7 and on day 6 cows were injected with luteolytic dose of PGF2? (d-cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m).Estrus response and estrus intensity are higher in CIDR group but ovulation rate is less in CIDR and high in PG and OVS group. Timing of ovulation is shortest in OVS group as compared to other groups. Pregnancy rate are also high in OVS group. Therefore it is concluded that Ovsynch protocol is helpful to improve ovulation and pregnancy rate in Sahiwal cows.
Availability: Items available for loan: UVAS Library [Call number: 1827,T] (1).
2.
Effect Of Trehalose On Cryopreservation Of Sahiwal Bull Spermatozoa
by Zohaib Ashraf | Dr. Aijaz ali channa | Prof .Dr. Aneela | Prof. DR. Nasim ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1948,T] (1).
3.
Effect Of Synchronization Protocols (Pg And Ovsynch) On Estrus Response, Estrus Intensity, Ovulation Time And Conception Rate In Cholistani Cows
by Muhammad Awais Ajmal (2009-VA-370) | Dr. Aijaz Ali Channa | Prof. Dr. Nasim Ahmad | Dr. Jawad Nazir.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cholistani cattle breed, like other milch breeds of our country (Sahiwal, Red Sindhi cattle), is one of the indigenous breeds of Pakistan having superior dairy characteristics. Being heat tolerant breed present in tropical and subtropical areas of Pakistan it has average milk production 1235 litters with 4.8% fat in it. To minimize the cross breeding of Cholistani cows, some efforts are being done for its conservation, through management and genetic improvement. Moreover genetic improvement, through artificial insemination (A.I) is in process. In recent times another important tool Fixed time artificial insemination (FTAI) has emerged to improve fertility which usually is performed in association with a variety of estrus and ovulation synchronization regimens. Synchronization of estrus is actually to bring large group of females in estrus at a desired fixed time by manipulation of estrus cycle. Two synchronization protocols were compared. In PG group, each animal was treated with luteolytic dose of PGF2α (d-Cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m) on random stage of the estrous cycle and repeated after 11 days TAI was done at 72 and 84 h after 2nd PG. In OVS group each cow received an intramuscular injection of gonadotropin releasing hormone (GnRH; 50 mcg of a GnRH analogue, Dalmarelin TM Fatro®, Italy; 2 ml; i.m) on random stage of estrus cycle (day 0). On day 7 these cows were treated with PGF2α (d-cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m), followed by second injection of GnRH on day 9 and TAI was done at 12 and 24h after 2nd GnRH. Estrus response and estrus intensity were higher in OVS group as compared to PG. Timing of ovulation was shorter in OVS group as compared to. Conception rate are also high in OVS group. Therefore it is concluded that Ovsynch protocol is helpful to improve ovulation and conception rate in Cholistani cows. Availability: Items available for loan: UVAS Library [Call number: 2523-T] (1).
4.
Relationship Among Physical Reproductive Charecters And Semen Quality Parameters At Different Age Groups Of Sahiwal Bulls
by Muhammad Asif (2015-VA-1077) | Dr. Aijaz Ali Channa | Prof. Dr. Nasim Ahmad | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The present study demonstrate the relationship among physical reproductive characters and
semen quality parameters at different age groups of Sahiwal bulls. The standard breeding
soundness evaluation criteria is not set for the screening of Sahiwal bulls. Artificial insemination
is a tool for rapid genetic improvement. Sahiwal bulls used as AI sire should be screened through
standard breeding soundness evaluation criteria. Therefore the present study has been designed to
determine objectives like: (1) to determine the relationship among physical reproductive characters
and semen quality parameters at different age groups of Sahiwal bulls (2) to compare the semen
quality parameters of pendulous with non-pendulous Sahiwal bulls. The study was carried out
during July-October 2016 at Semen Production Unit Qadirabad district Sahiwal. The Sahiwal bulls
(n=91) maintained at SPU as a regular semen donor bulls, having no reproductive disorder were
selected for this study. These bulls were divided into four different age groups G1 (24-48 months,
n=8), G2 (49-72 months, n=50), G3 (73-96 months, n=27) and G4 (97-108, n=6). The evaluation
of the bulls were done for BW, SC and DPS. Semen quality parameters (ejaculate volume, sperm
concentration, motility, percent live sperm and morphological abnormalities) were evaluated and
compare between different groups. Mean BW and SC increases (P˂ 0.05) with the age of bulls,
but DPS is not correlated with the age means it does not increases with the age. Ejaculate volume
(3.58±0.1, 3.92±.05, 3.97±.06, 4.24±0.15 ml), sperm concentaration (870.6±14.1, 897.4±6.5,
899.5± 8.0, 856.9±20.2 x 10x6 /ml) increases with age. However motility remain more or less
unchange with age . The percentage of live sperms were 75.06±0.3, 75.75±0.1, 75.96±0.1,
77.09±0.4 respectively and percentage of morphological abnormalities were
Summary
32
19.57±0.6,17.61±0.2,15.80±0.2,16.69±0.4 repectively. The morphological abnormalities were
higher in younger bulls as compered to older bulls. It can be concluded that the BW, SC, ejaculate
volume, sperm concentration (except in G4 due to small sample size) increases as that the age
increases, whereas the other semen quality parameters like individual motility, motility after
dilution and individual motility after freezing looks to be more or less independent of age. The
DPS is not correlated with the age, it may be a genetic character. In comparison of semen quality
between pendulous and non-pendulous bulls, the ejaculate volume and sperm concentration were
significantly higher in pendulous bulls instead of non-pendulous bulls (in G2 and G3) and nonsignificantly
higher (in G1 and G4). While comparing the other semen parameter like motility
percentage (individual, after dilution and after freezing) between pendulous and non-pendulous
bulls the results remain non-significant. The findings of this study direct us to focus more on nonpendulous
bulls instead of pendulous bulls, the reason behind that is the pendulous bull’s needs
extra managemental care as they are more prone to injuries and perpetual infections. These
findings could be used to set the selection criteria for Sahiwal bulls. Availability: Items available for loan: UVAS Library [Call number: 2922-T] (1).
